Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements.

ABSTRACT: Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio-temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two cis-regulatory functions are thought to operate through distinct dedicated elements. By examining the occupancy of the Drosophila pleiohomeotic repressive complex (PhoRC) during embryogenesis, we revealed extensive co-occupancy at developmental enhancers. Using an established in vivo assay for PRE activity, we demonstrated that a subset of characterized developmental enhancers can function as PREs, silencing transcription in a Polycomb-dependent manner. Conversely, some classic Drosophila PREs can function as developmental enhancers in vivo, activating spat

SUBMITTER: Erceg J 

PROVIDER: S-EPMC5393054 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature