Pseudouridine and N⁶-methyladenosine modifications weaken PUF protein/RNA interactions.

ABSTRACT: RNA modifications are ubiquitous in biology, with over 100 distinct modifications. While the vast majority were identified and characterized on abundant noncoding RNA such as tRNA and rRNA, the advent of sensitive sequencing-based approaches has led to the discovery of extensive and regulated modification of eukaryotic messenger RNAs as well. The two most abundant mRNA modifications-pseudouridine (Ψ) and N⁶-methyladenosine (m⁶A)-affect diverse cellular processes including mRNA splicing, localization, translation, and decay and modulate RNA structure. Here, we test the hypothesis that RNA modifications directly affect interactions between RNA-binding proteins and target RNA. We show that Ψ and m⁶A weaken the binding of the human single-stranded RNA bi

SUBMITTER: Vaidyanathan PP 

PROVIDER: S-EPMC5393172 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature