The structure of an E. coli tRNA_f^Met A₁-U₇₂ variant shows an unusual conformation of the A₁-U₇₂ base pair.

ABSTRACT: Translation initiation in eukaryotes and archaea involves a methionylated initiator tRNA delivered to the ribosome in a ternary complex with e/aIF2 and GTP. Eukaryotic and archaeal initiator tRNAs contain a highly conserved A₁-U₇₂ base pair at the top of the acceptor stem. The importance of this base pair to discriminate initiator tRNAs from elongator tRNAs has been established previously using genetics and biochemistry. However, no structural data illustrating how the A₁-U₇₂ base pair participates in the accurate selection of the initiator tRNAs by the translation initiation systems are available. Here, we describe the crystal structure of a mutant E. coli initiator tRNA_f^MetA₁-U₇₂, aminoacylated

SUBMITTER: Monestier A 

PROVIDER: S-EPMC5393177 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature