Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases.

ABSTRACT: The kinetics of hydrogen oxidation and evolution by [FeFe]-hydrogenases have been investigated by electrochemical impedance spectroscopy-resolving factors that determine the exceptional activity of these enzymes, and introducing an unusual and powerful way of analyzing their catalytic electron transport properties. Attached to an electrode, hydrogenases display reversible electrocatalytic behavior close to the 2H⁺/H₂ potential, making them paradigms for efficiency: the electrocatalytic "exchange" rate (measured around zero driving force) is therefore an unusual parameter with theoretical and practical significance. Experiments were carried out on two [FeFe]-hydrogenases, CrHydA1 from the green alga Chlamydomonas reinhardtii, which contains only the activ

SUBMITTER: Pandey K 

PROVIDER: S-EPMC5393252 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature