Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II.

ABSTRACT:

Background

Down-regulation of plasma membrane receptors via the endocytic pathway involves their monoubiquitylation, transport to endosomal membranes and eventual sorting into multi vesicular bodies (MVB) destined for lysosomal degradation. Successive assemblies of Endosomal Sorting Complexes Required for Transport (ESCRT-I, -II and III) largely mediate sorting of plasma membrane receptors at endosomal membranes, the formation of multivesicular bodies and their release into the endosomal lumen. In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity.

Results

Here we report the crystal structure of Vps25 at 3.1 A resolution. Vps25 crystallizes in a dimeric form and each mo

SUBMITTER: Wernimont AK 

PROVIDER: S-EPMC539351 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature