In Situ Molecular Architecture of the Salmonella Type III Secretion Machine.

ABSTRACT: Type III protein secretion systems have specifically evolved to deliver bacterially encoded proteins into target eukaryotic cells. The core elements of this multi-protein machine are the envelope-associated needle complex, the inner membrane export apparatus, and a large cytoplasmic sorting platform. Here, we report a high-resolution in situ structure of the Salmonella Typhimurium type III secretion machine obtained by high-throughput cryo-electron tomography and sub-tomogram averaging. Through molecular modeling and comparative analysis of machines assembled with protein-tagged components or from different deletion mutants, we determined the molecular architecture of the secretion machine in situ and localized its structural components. We also show that docking of the sorting platform re

SUBMITTER: Hu B 

PROVIDER: S-EPMC5393631 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature