Chromatin Domain Organization of the TCRb Locus and Its Perturbation by Ectopic CTCF Binding.

ABSTRACT: CTCF-mediated chromatin interactions influence organization and function of mammalian genome in diverse ways. We analyzed the interactions among CTCF binding sites (CBS) at the murine TCRb locus to discern the role of CTCF-mediated interactions in the regulation of transcription and VDJ recombination. Chromosome conformation capture analysis revealed thymocyte-specific long-range intrachromosomal interactions among various CBS across the locus that were relevant for defining the limit of the enhancer Eb-regulated recombination center (RC) and for facilitating the spatial proximity of TCRb variable (V) gene segments to the RC. Ectopic CTCF binding in the RC region, effected via genetic manipulation, altered CBS-directed chromatin loops, interfered with RC establishment, and reduced the spat

SUBMITTER: Rawat P 

PROVIDER: S-EPMC5394274 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature