Use of Natural Transformation To Establish an Easy Knockout Method in Riemerella anatipestifer.

ABSTRACT: Riemerella anatipestifer is a member of the family Flavobacteriaceae and a major causative agent of duck serositis. Little is known about its genetics and pathogenesis. Several bacteria are competent for natural transformation; however, whether R. anatipestifer is also competent for natural transformation has not been investigated. Here, we showed that R. anatipestifer strain ATCC 11845 can uptake the chromosomal DNA of R. anatipestifer strain RA-CH-1 in all growth phases. Subsequently, a natural transformation-based knockout method was established for R. anatipestifer ATCC 11845. Targeted mutagenesis gave transformation frequencies of ∼10^-5 transformants. Competition assay experiments showed that R. anatipestifer ATCC 11845 prefe

SUBMITTER: Liu M 

PROVIDER: S-EPMC5394337 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature