Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice.

ABSTRACT:

Background

A lesion-mimic mutant in rice (Oryza sativa L.), spotted leaf 5 (spl5), displays a disease-resistance-enhanced phenotype, indicating that SPL5 negatively regulates cell death and resistance responses. To understand the molecular mechanisms of SPL5 mutation-induced cell death and resistance responses, a proteomics-based approach was used to identify differentially accumulated proteins between the spl5 mutant and wild type (WT).

Results

Proteomic data from two-dimensional gel electrophoresis showed that 14 candidate proteins were significantly up- or down-regulated in the spl5 mutant compared with WT. These proteins are involved in diverse biological processes including pre-mRNA splicing, amino acid metabolism, photosynthesis, glycolysis, reactive oxygen species (RO

SUBMITTER: Chen X 

PROVIDER: S-EPMC5394886 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature