Rapid inducible protein displacement in Plasmodiumin vivo and in vitro using knocksideways technology.

ABSTRACT: A deeper understanding of the biology of the Plasmodium parasite is essential in order to identify targets for interventions, with the ultimate aim of eliminating malaria. Determining the function(s) of essential proteins in Plasmodium has, until recently, been hampered by the lack of efficient conditional systems to abrogate proteins. We report the adaptation of a conditional technology, knocksideways (KS), for use in Plasmodium berghei, which can potentially rapidly inactivate proteins of interest through relocalisation. The system is induced using rapamycin, which allows for KS both in vitro and in vivo and is effective more rapidly than any other reported system. KS utilises pairs of fluorescent tags that facilitate live imaging and allows for rapid c

SUBMITTER: Hughes KR 

PROVIDER: S-EPMC5395084 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature