Unraveling the Composition and Behavior of Heterogeneous Lipid Nanodiscs by Mass Spectrometry.

ABSTRACT: Mass spectrometry (MS) has emerged as a powerful tool to study membrane protein complexes and protein-lipid interactions. Because they provide a precisely defined lipid bilayer environment, lipoprotein Nanodiscs offer a promising cassette for membrane protein MS analysis. However, heterogeneous lipids create several potential challenges for native MS: additional spectral complexity, ambiguous assignments, and differing gas-phase behaviors. Here, we present strategies to address these challenges and streamline analysis of heterogeneous-lipid Nanodiscs. We show that using two lipids of similar mass limits the complexity of the spectra in heterogeneous Nanodiscs and that the lipid composition can be determined by using a dual Fourier transform approach to obtain the average lipid mass. Furthe

SUBMITTER: Hoi KK 

PROVIDER: S-EPMC5395085 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature