Multidrug-resistant bacteria compensate for the epistasis between resistances.

ABSTRACT: Mutations conferring resistance to antibiotics are typically costly in the absence of the drug, but bacteria can reduce this cost by acquiring compensatory mutations. Thus, the rate of acquisition of compensatory mutations and their effects are key for the maintenance and dissemination of antibiotic resistances. While compensation for single resistances has been extensively studied, compensatory evolution of multiresistant bacteria remains unexplored. Importantly, since resistance mutations often interact epistatically, compensation of multiresistant bacteria may significantly differ from that of single-resistant strains. We used experimental evolution, next-generation sequencing, in silico simulations, and genome editing to compare the compensatory process of a streptomycin and rifampicin

SUBMITTER: Moura de Sousa J 

PROVIDER: S-EPMC5395140 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature