PKC-epsilon and TLR4 synergistically regulate resistin-mediated inflammation in human macrophages.

ABSTRACT:

Background and aims

Resistin has been associated with atherosclerotic inflammation and cardiovascular complications. We and others have previously shown that PKC-epsilon (PKCε) is involved in resistin-induced smooth muscle cell (VSMC) dysfunction at a high pathological concentration. This study aimed to evaluate the role and potential pathways of resistin at a physiological concentration, in atherosclerosis-related inflammation.

Methods

Plasma from patients with atherosclerosis was analyzed for resistin concentration. Patients were divided into tertiles based on resistin levels and cytokines were compared between tertiles. Macrophages were then treated with resistin in the presence or absence of PKCε inhibitor and/or TLR4 blocking-antibody, and their inflammatory state was e

SUBMITTER: Zuniga MC 

PROVIDER: S-EPMC5395299 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature