TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.

ABSTRACT: The resolution and formation of facultative heterochromatin are essential for development, reprogramming, and oncogenesis. The mechanisms underlying these changes are poorly understood owing to the difficulty of studying heterochromatin dynamics and structure in vivo. We devised an in vivo approach to investigate these mechanisms and found that topoisomerase II (TOP2), but not TOP1, synergizes with BAF (mSWI/SNF) ATP-dependent chromatin remodeling complexes genome-wide to resolve facultative heterochromatin to accessible chromatin independent of transcription. This indicates that changes in DNA topology that take place through (de-)catenation rather than the release of torsional stress through swiveling are necessary for heterochromatin resolution. TOP2 and BAF cooperate to recruit pluripo

SUBMITTER: Miller EL 

PROVIDER: S-EPMC5395302 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature