Host conditioning and rejection monitoring in hepatocyte transplantation in humans.

ABSTRACT:

Background & aims

Hepatocyte transplantation partially corrects genetic disorders and has been associated anecdotally with reversal of acute liver failure. Monitoring for graft function and rejection has been difficult, and has contributed to limited graft survival. Here we aimed to use preparative liver-directed radiation therapy, and continuous monitoring for possible rejection in an attempt to overcome these limitations.

Methods

Preparative hepatic irradiation was examined in non-human primates as a strategy to improve engraftment of donor hepatocytes, and was then applied in human subjects. T cell immune monitoring was also examined in human subjects to assess adequacy of immunosuppression.

Results

Porcine hepatocyte transplants engrafted and expanded to comprise

SUBMITTER: Soltys KA 

PROVIDER: S-EPMC5395353 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature