Molecular and Morphometrical Characterization of Fasciola Species Isolated from Domestic Ruminants in Ardabil Province, Northwestern Iran.

ABSTRACT:

Background

We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran.

Methods

Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms. Statistical analysis was conducted using the Student's t-test implemented in SPSS 15.0 (SPSS, Chicago, Illinois). Then the morphometric criteria of Fasciola samples were compared with PCR-R

SUBMITTER: Aryaeipour M 

PROVIDER: S-EPMC5395527 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature