Immune Responses to Broad-Spectrum Antibiotic Treatment and Fecal Microbiota Transplantation in Mice.

ABSTRACT: Compelling evidence demonstrates the pivotal role of the commensal intestinal microbiota in host physiology and the detrimental effects of its perturbations following antibiotic treatment. Aim of this study was to investigate the impact of antibiotics induced depletion and subsequent restoration of the intestinal microbiota composition on the murine mucosal and systemic immunity. To address this, conventional C57BL/6j mice were subjected to broad-spectrum antibiotic treatment for 8 weeks. Restoration of the intestinal microbiota by peroral fecal microbiota transplantation (FMT) led to reestablishment of small intestinal CD4⁺, CD8⁺, and B220⁺ as well as of colonic CD4⁺ cell numbers as early as 7 days post-FMT. However, at d28 following FMT, coloni

SUBMITTER: Ekmekciu I 

PROVIDER: S-EPMC5395657 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature