Leucine-rich-repeat-containing variable lymphocyte receptors as modules to target plant-expressed proteins.

ABSTRACT:

Background

The ability to target and manipulate protein-based cellular processes would accelerate plant research; yet, the technology to specifically and selectively target plant-expressed proteins is still in its infancy. Leucine-rich repeats (LRRs) are ubiquitously present protein domains involved in mediating protein-protein interactions. LRRs confer the binding specificity to the highly diverse variable lymphocyte receptor (VLR) antibodies (including VLRA, VLRB and VLRC types) that jawless vertebrates make as the functional equivalents of jawed vertebrate immunoglobulin-based antibodies.

Results

In this study, VLRBs targeting an effector protein from a plant pathogen, HopM1, were developed by immunizing lampreys and using yeast surface display to select for high-affinity

SUBMITTER: Velasquez AC 

PROVIDER: S-EPMC5395774 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature