Assessment of metabolic phenotypic variability in children's urine using ¹H NMR spectroscopy.

ABSTRACT: The application of metabolic phenotyping in clinical and epidemiological studies is limited by a poor understanding of inter-individual, intra-individual and temporal variability in metabolic phenotypes. Using ¹H NMR spectroscopy we characterised short-term variability in urinary metabolites measured from 20 children aged 8-9 years old. Daily spot morning, night-time and pooled (50:50 morning and night-time) urine samples across six days (18 samples per child) were analysed, and 44 metabolites quantified. Intraclass correlation coefficients (ICC) and mixed effect models were applied to assess the reproducibility and biological variance of metabolic phenotypes. Excellent analytical reproducibility and precision was demonstrated for the ¹H NMR spectroscopic platform (me

SUBMITTER: Maitre L 

PROVIDER: S-EPMC5395814 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature