Sensitive single-molecule protein quantification and protein complex detection in a microarray format.

ABSTRACT: Single-molecule protein analysis provides sensitive protein quantitation with a digital read-out and is promising for studying biological systems and detecting biomarkers clinically. However, current single-molecule platforms rely on the quantification of one protein at a time. Conventional antibody microarrays are scalable to detect many proteins simultaneously, but they rely on less sensitive and less quantitative quantification by the ensemble averaging of fluorescent molecules. Here, we demonstrate a single-molecule protein assay in a microarray format enabled by an ultra-low background surface and single-molecule imaging. The digital read-out provides a highly sensitive, low femtomolar limit of detection and four orders of magnitude of dynamic range through the use of hybrid digital-a

SUBMITTER: Tessler LA 

PROVIDER: S-EPMC5396177 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature