Mast cells are permissive for rhinovirus replication: potential implications for asthma exacerbations.

ABSTRACT:

Background

Human rhinoviruses (HRVs) are a major trigger of asthma exacerbations, with the bronchial epithelium being the major site of HRV infection and replication. Mast cells (MCs) play a key role in asthma where their numbers are increased in the bronchial epithelium with increasing disease severity.

Objective

In view of the emerging role of MCs in innate immunity and increased localization to the asthmatic bronchial epithelium, we investigated whether HRV infection of MCs generated innate immune responses which were protective against infection.

Methods

The LAD2 MC line or primary human cord blood-derived MCs (CBMCs) were infected with HRV or UV-irradiated HRV at increasing multiplicities of infection (MOI) without or with IFN-β or IFN-λ. After 24 h, innate immun

SUBMITTER: Akoto C 

PROVIDER: S-EPMC5396281 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature