Na⁺ /H⁺ exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells.

ABSTRACT: Following superficial injury, neighbouring gastric epithelial cells close the wound by rapid cell migration, a process called epithelial restitution. Na⁺ /H⁺ exchange (NHE) inhibitors interfere with restitution, but the role of the different NHE isoforms expressed in gastric pit cells has remained elusive. The role of the basolaterally expressed NHE1 (Slc9a1) and the presumably apically expressed NHE2 (Slc9a2) in epithelial restitution was investigated in the nontransformed rat gastric surface cell line RGM1. Migration velocity was assessed by loading the cells with the fluorescent dye DiR and following closure of an experimental wound over time. Since RGM1 cells expressed very low NHE2 mRNA and have low transport activity, NHE2 was introduced by lentiviral gene trans

SUBMITTER: Paehler Vor der Nolte A 

PROVIDER: S-EPMC5396337 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature