Simultaneous impedance spectroscopy and fluorescence microscopy for the real-time monitoring of the response of cells to drugs.

ABSTRACT: A dual fluorescence microscopy and electrochemical strategy to investigate how cell-surface interactions influence the cellular responses to cues for the cell-based biosensing of drug efficacy is reported herein. The combined method can be used to not only monitor the importance of controlling the cellular adhesive environment on the cell response to drugs but it also provides biological information on the timescales of downstream outside-in signaling from soluble cues. As an example of the use of the combined method, we show how adhesive cues influence the signalling responses of cells to soluble cues. G-protein-coupled receptors were used as the target for the soluble cues. The changes in cell adhesion, cell morphology and Ca²⁺ flux induced by soluble histamine were simultaneo

SUBMITTER: Parviz M 

PROVIDER: S-EPMC5396555 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature