Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide.

ABSTRACT: The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO₂, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitosan sequentially activated with Glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction (1: 1 (alcohol-acid); biocatalyst content, 10% (by substrate mass); 45 °C), an experimental design (2³) was conducted to evaluate the effects of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum loading of chitosan was 20 mg protein/g support,

SUBMITTER: Dos Santos JCS 

PROVIDER: S-EPMC5397107 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature