The impact of base stacking on the conformations and electrostatics of single-stranded DNA.

ABSTRACT: Single-stranded DNA (ssDNA) is notable for its interactions with ssDNA binding proteins (SSBs) during fundamentally important biological processes including DNA repair and replication. Previous work has begun to characterize the conformational and electrostatic properties of ssDNA in association with SSBs. However, the conformational distributions of free ssDNA have been difficult to determine. To capture the vast array of ssDNA conformations in solution, we pair small angle X-ray scattering with novel ensemble fitting methods, obtaining key parameters such as the size, shape and stacking character of strands with different sequences. Complementary ion counting measurements using inductively coupled plasma atomic emission spectroscopy are employed to determine the composition of the ion at

SUBMITTER: Plumridge A 

PROVIDER: S-EPMC5397193 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature