DNA replication-timing analysis of human chromosome 22 at high resolution and different developmental states.

ABSTRACT: Duplication of the genome during the S phase of the cell cycle does not occur simultaneously; rather, different sequences are replicated at different times. The replication timing of specific sequences can change during development; however, the determinants of this dynamic process are poorly understood. To gain insights into the contribution of developmental state, genomic sequence, and transcriptional activity to replication timing, we investigated the timing of DNA replication at high resolution along an entire human chromosome (chromosome 22) in two different cell types. The pattern of replication timing was correlated with respect to annotated genes, gene expression, novel transcribed regions of unknown function, sequence composition, and cytological features. We observed that chromos

SUBMITTER: White EJ 

PROVIDER: S-EPMC539744 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature