Label-free single-cell separation and imaging of cancer cells using an integrated microfluidic system.

ABSTRACT: The incidence of cancer is increasing worldwide and metastatic disease, through the spread of circulating tumor cells (CTCs), is responsible for the majority of the cancer deaths. Accurate monitoring of CTC levels in blood provides clinical information supporting therapeutic decision making, and improved methods for CTC enumeration are asked for. Microfluidics has been extensively used for this purpose but most methods require several post-separation processing steps including concentration of the sample before analysis. This induces a high risk of sample loss of the collected rare cells. Here, an integrated system is presented that efficiently eliminates this risk by integrating label-free separation with single cell arraying of the target cell population, enabling direct on-chip tumor ce

SUBMITTER: Antfolk M 

PROVIDER: S-EPMC5397835 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature