Plant gum identification in historic artworks.

ABSTRACT: We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage. Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis. We developed an enzyme cocktail suitable for plant gums of unknown composition. Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified. A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively Pent_xHex_y and Hex_n profiles. Optimized for low sample quantities, the analytical protocol was

SUBMITTER: Granzotto C 

PROVIDER: S-EPMC5397872 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature