¹H NMR Shows Slow Phospholipid Flip-Flop in Gel and Fluid Bilayers.

ABSTRACT: We measured the transbilayer diffusion of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in large unilamellar vesicles, in both the gel (L_β') and fluid (L_α) phases. The choline resonance of headgroup-protiated DPPC exchanged into the outer leaflet of headgroup-deuterated DPPC-d13 vesicles was monitored using ¹H NMR spectroscopy, coupled with the addition of a paramagnetic shift reagent. This allowed us to distinguish between the inner and outer bilayer leaflet of DPPC, to determine the flip-flop rate as a function of temperature. Flip-flop of fluid-phase DPPC exhibited Arrhenius kinetics, from which we determined an activation energy of 122 kJ mol^-1. In gel-phase DPPC vesicles, flip-flop was not observed over the course of 250 h. Our findings

SUBMITTER: Marquardt D 

PROVIDER: S-EPMC5397887 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature