Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics.

ABSTRACT:

Background

Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is necessary to differentiate the true negative results from those consequent from a failure in some step of the nucleic acid test.

Objectives

The aim of this study was the construction of virus-modified particles, based on MS2 bacteriophage, to be used as IC for the diagnosis of RNA viruses.

Methods

The MS2 genome was cloned into the pET47b(+) plasmid, generating pET47b(+)-MS2. MS2-like particles were produced through

SUBMITTER: Zambenedetti MR 

PROVIDER: S-EPMC5398160 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature