Analysis of host microRNA function uncovers a role for miR-29b-2-5p in Shigella capture by filopodia.

ABSTRACT: MicroRNAs play an important role in the interplay between bacterial pathogens and host cells, participating as host defense mechanisms, as well as exploited by bacteria to subvert host cellular functions. Here, we show that microRNAs modulate infection by Shigella flexneri, a major causative agent of bacillary dysentery in humans. Specifically, we characterize the dual regulatory role of miR-29b-2-5p during infection, showing that this microRNA strongly favors Shigella infection by promoting both bacterial binding to host cells and intracellular replication. Using a combination of transcriptome analysis and targeted high-content RNAi screening, we identify UNC5C as a direct target of miR-29b-2-5p and show its pivotal role in the modulation of Shigella binding to host cells. MiR-29b-2-5p, t

SUBMITTER: Sunkavalli U 

PROVIDER: S-EPMC5398735 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature