Expression Profiling of Nonpolar Lipids in Meibum From Patients With Dry Eye: A Pilot Study.

ABSTRACT:

Purpose

The purpose of this investigation was to characterize differentially expressed lipids in meibum samples from patients with dry eye disease (DED) in order to better understand the underlying pathologic mechanisms.

Methods

Meibum samples were collected from postmenopausal women with DED (PW-DED; n = 5) and a control group of postmenopausal women without DED (n = 4). Lipid profiles were analyzed by direct infusion full-scan electrospray ionization mass spectrometry (ESI-MS). An initial analysis of 145 representative peaks from four classes of lipids in PW-DED samples revealed that additional manual corrections for peak overlap and isotopes only slightly affected the statistical analysis. Therefore, analysis of uncorrected data, which can be applied to a greater number o

SUBMITTER: Chen J 

PROVIDER: S-EPMC5398788 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature