HIV-1 and SIV Predominantly Use CCR5 Expressed on a Precursor Population to Establish Infection in T Follicular Helper Cells.

ABSTRACT:

Background

T follicular helper (Tfh) cells are increasingly recognized as a major reservoir of HIV infection that will likely need to be addressed in approaches to curing HIV. However, Tfh express minimal CCR5, the major coreceptor for HIV-1, and the mechanism by which they are infected is unclear. We have previously shown that macaque Tfh lack CCR5, but are infected in vivo with CCR5-using SIV at levels comparable to other memory CD4⁺ T cells. Similarly, human splenic Tfh cells are highly infected with HIV-1 DNA. Therefore, we set out to examine the mechanism of infection of Tfh cells.

Methodology

Tfh and other CD4⁺ T cell subsets from macaque lymph nodes and spleens, splenic Tfh from HIV⁺ subjects, and tonsillar Tfh from HIV-uninfect

SUBMITTER: Xu Y 

PROVIDER: S-EPMC5399036 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature