Conserved functions of the trigger loop and Gre factors in RNA cleavage by bacterial RNA polymerases.

ABSTRACT: RNA cleavage by RNA polymerase (RNAP) is the central step in co-transcriptional RNA proofreading. Bacterial RNAPs were proposed to rely on the same mobile element of the active site, the trigger loop (TL), for both nucleotide addition and RNA cleavage. RNA cleavage can also be stimulated by universal Gre factors, which should replace the TL to get access to the RNAP active site. The contributions of the TL and Gre factors to RNA cleavage reportedly vary between RNAPs from different bacterial species and, probably, different types of transcription complexes. Here, by comparing RNAPs from Escherichia coli, Deinococcus radiodurans, and Thermus aquaticus, we show that the functions of the TL and Gre factors in RNA cleavage are conserved in various species, with important v

SUBMITTER: Miropolskaya N 

PROVIDER: S-EPMC5399121 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature