Molecular determinants of the N-terminal acetyltransferase Naa60 anchoring to the Golgi membrane.

ABSTRACT: Nα-Acetyltransferase 60 (Naa60 or NatF) was recently identified as an unconventional N-terminal acetyltransferase (NAT) because it localizes to organelles, in particular the Golgi apparatus, and has a preference for acetylating N termini of the transmembrane proteins. This knowledge challenged the prevailing view of N-terminal acetylation as a co-translational ribosome-associated process and suggested a new mechanistic functioning for the enzymes responsible for this increasingly recognized protein modification. Crystallography studies on Naa60 were unable to resolve the C-terminal tail of Naa60, which is responsible for the organellar localization. Here, we combined modeling, in vitro assays, and cellular localization studies to investigate the secondary structure and membra

SUBMITTER: Aksnes H 

PROVIDER: S-EPMC5399128 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature