Synthesis and hyperpolarisation of eNOS substrates for quantification of NO production by ¹H NMR spectroscopy.

ABSTRACT: Hyperpolarization enhances the intensity of the NMR signals of a molecule, whose in vivo metabolic fate can be monitored by MRI with higher sensitivity. SABRE is a hyperpolarization technique that could potentially be used to image nitric oxide (NO) production in vivo. This would be very important, because NO dysregulation is involved in several pathologies, including cardiovascular ones. The nitric oxide synthase (NOS) pathway leads to NO production via conversion of l-arginine into l-citrulline. NO is a free radical gas with a short half-life in vivo (≈5s), therefore direct NO quantification is challenging. An indirect method - based on quantifying conversion of an l-Arg- to l-Cit-derivative by ¹H NMR spectroscopy - is herein proposed. A small library of pyridyl containing l-A

SUBMITTER: Fernandez Diaz-Rullo F 

PROVIDER: S-EPMC5399308 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature