TNF-α promotes extracellular vesicle release in mouse astrocytes through glutaminase.

ABSTRACT:

Background

Extracellular vesicles (EVs) are membrane-contained vesicles shed from cells. EVs contain proteins, lipids, and nucleotides, all of which play important roles in intercellular communication. The release of EVs is known to increase during neuroinflammation. Glutaminase, a mitochondrial enzyme that converts glutamine to glutamate, has been implicated in the biogenesis of EVs. We have previously demonstrated that TNF-α promotes glutaminase expression in neurons. However, the expression and the functionality of glutaminase in astrocytes during neuroinflammation remain unknown. We posit that TNF-α can promote the release of EVs in astrocytes through upregulation of glutaminase expression.

Results

Release of EVs, which was demonstrated by electron microscopy, nanopartic

SUBMITTER: Wang K 

PROVIDER: S-EPMC5399318 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature