Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya.

ABSTRACT:

Background

Zika, dengue, and chikungunya are three mosquito-borne viruses having overlapping transmission vectors. They cause diseases having similar symptoms in human patients, but requiring different immediate management steps. Therefore, rapid (< one hour) discrimination of these three viruses in patient samples and trapped mosquitoes is needed. The need for speed precludes any assay that requires complex up-front sample preparation, such as extraction of nucleic acids from the sample. Also precluded in robust point-of-sampling assays is downstream release of the amplicon mixture, as this risks contamination of future samples that will give false positives.

Methods

Procedures are reported that directly test urine and plasma (for patient diagnostics) or crushed mosquito ca

SUBMITTER: Yaren O 

PROVIDER: S-EPMC5399334 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature