Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.

ABSTRACT: Goosegrass (Eleusine indica) is one of the most serious annual grassy weeds worldwide, and its evolved herbicide-resistant populations are more difficult to control. Quantitative real-time PCR (qPCR) is a common technique for investigating the resistance mechanism; however, there is as yet no report on the systematic selection of stable reference genes for goosegrass. This study proposed to test the expression stability of 9 candidate reference genes in goosegrass in different tissues and developmental stages and under stress from three types of herbicide. The results show that for different developmental stages and organs (control), eukaryotic initiation factor 4 A (eIF-4) is the most stable reference gene. Chloroplast acetolactate synthase (ALS) is the most stable reference gene under gl

SUBMITTER: Chen J 

PROVIDER: S-EPMC5399354 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature