Comparative genomics and evolution of the amylase-binding proteins of oral streptococci.

ABSTRACT:

Background

Successful commensal bacteria have evolved to maintain colonization in challenging environments. The oral viridans streptococci are pioneer colonizers of dental plaque biofilm. Some of these bacteria have adapted to life in the oral cavity by binding salivary α-amylase, which hydrolyzes dietary starch, thus providing a source of nutrition. Oral streptococcal species bind α-amylase by expressing a variety of amylase-binding proteins (ABPs). Here we determine the genotypic basis of amylase binding where proteins of diverse size and function share a common phenotype.

Results

ABPs were detected in culture supernatants of 27 of 59 strains representing 13 oral Streptococcus species screened using the amylase-ligand binding assay. N-terminal sequences from ABPs of divers

SUBMITTER: Haase EM 

PROVIDER: S-EPMC5399409 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature