In-depth characterization of the microRNA transcriptome in normal thyroid and papillary thyroid carcinoma.

ABSTRACT:

Context

A single microRNA gene may give rise to several mature products that differ in length, called isomiRs. IsomiRs are known to be tissue specific and functionally relevant. The microRNA sequence heterogeneity of the thyroid gland has yet to be determined.

Objective

The objective of the study was to provide a comprehensive view of the microRNA transcriptome in normal thyroid and papillary thyroid carcinoma (PTC).

Design

We used next-generation deep sequencing to analyze microRNA length heterogeneity and expression profiles of PTC tumors (n = 14), unaffected tissue adjacent to tumors (n = 14), and control, noncancerous thyroid tissue (n = 14). The results were validated with a microarray on an additional set of 9 PTC tumor/normal tissue pairs.

Results

Eighty

SUBMITTER: Swierniak M 

PROVIDER: S-EPMC5399477 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature