Molecular characterization of an unauthorized genetically modified Bacillus subtilis production strain identified in a vitamin B₂ feed additive.

ABSTRACT: Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed and are often used. The presence of neither the GM strain nor its recombinant DNA is allowed for fermentation products placed on the EU market as food or feed additive. A vitamin B₂ product (80% feed grade) imported from China was analysed. Viable B. subtilis cells were identified and DNAs of two bacterial isolates (LHL and LGL) were subjected to three whole genome sequencing (WGS) runs with different devices (MiSeq, 454 or HiSeq system). WGS data revealed the integration of a chloramphenicol resistance gene, the deletion of the endogenous riboflavin (rib) operon and presence of four putative p

SUBMITTER: Paracchini V 

PROVIDER: S-EPMC5399532 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature