The Regenerative Potential of Notochordal Cells in a Nucleus Pulposus Explant.

ABSTRACT:

Study design

In vitro disk explant culture.

Objective

Notochordal cells (NCs) have been shown to upregulate matrix production by nucleus pulposus (NP) cells in coculture. To examine the translation of these in vitro results to a nativelike setting, the regenerative potential of NCs injected into NP tissue was assessed in this study.

Methods

NP explants were cultured after injection with NCs in phosphate-buffered saline (PBS) or with PBS alone (sham). At days 0 and 42, cell viability and morphology, water, DNA, sulfated glycosaminoglycan and hydroxyproline content, and gene expression of anabolic markers were analyzed.

Results

NCs remained viable during culture, but their morphology changed. The biochemical content remained unchanged, except for the DNA content

SUBMITTER: Arkesteijn ITM 

PROVIDER: S-EPMC5400162 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature