Conditional genetic deletion of Ano1 in interstitial cells of Cajal impairs Ca²⁺ transients and slow waves in adult mouse small intestine.

ABSTRACT: Myenteric plexus interstitial cells of Cajal (ICC-MY) in the small intestine are Kit⁺ electrical pacemakers that express the Ano1/TMEM16A Ca²⁺-activated Cl^- channel, whose functions in the gastrointestinal tract remain incompletely understood. In this study, an inducible Cre-LoxP-based approach was used to advance the understanding of Ano1 in ICC-MY of adult mouse small intestine. Kit^CreERT2/+;Ano1^Fl/Fl mice were treated with tamoxifen or vehicle, and small intestines (mucosa free) were examined. Quantitative RT-PCR demonstrated ~50% reduction in Ano1 mRNA in intestines of conditional knockouts (cKOs) compared with vehicle-treated controls. Whole mount immunohistochemistry showed a mosaic/patchy pattern loss of Ano1 protein in I

SUBMITTER: Malysz J 

PROVIDER: S-EPMC5401988 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature