Genome adaptive evolution of Lactobacillus casei under long-term antibiotic selection pressures.

ABSTRACT:

Background

The extensive use of antibiotics in medicine has raised serious concerns about biosafety. However, the effect of antibiotic application on the adaptive evolution of microorganisms, especially to probiotic bacteria, has not been well characterized. Thus, the objective of the current work was to investigate how antibiotic selection forces might drive genome adaptation using Lactobacillus (L.) casei Zhang as a model.

Methods

Two antibiotics, amoxicillin and gentamicin, were consistently applied to the laboratory culture of L. casei Zhang. We then monitored the mutations in the bacterial genome and changes in the minimum inhibitory concentrations (MICs) of these two antibiotics along a 2000-generation-cultivation lasted over 10 months.

Results

We found an appro

SUBMITTER: Wang J 

PROVIDER: S-EPMC5402323 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature