Physiologically Based Simulations of Deuterated Glucose for Quantifying Cell Turnover in Humans.

ABSTRACT: In vivo [6,6-²H₂]-glucose labeling is a state-of-the-art technique for quantifying cell proliferation and cell disappearance in humans. However, there are discrepancies between estimates of T cell proliferation reported in short (1-day) versus long (7-day) ²H₂-glucose studies and very-long (9-week) ²H₂O studies. It has been suggested that these discrepancies arise from underestimation of true glucose exposure from intermittent blood sampling in the 1-day study. Label availability in glucose studies is normally approximated by a "square pulse" (Sq pulse). Since the body glucose pool is small and turns over rapidly, the availability of labeled glucose can be subject to large fluctuations and the Sq pulse approximation m

SUBMITTER: Lahoz-Beneytez J 

PROVIDER: S-EPMC5403812 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature