Non-fused Phospholes as Fluorescent Probes for Imaging of Lipid Droplets in Living Cells.

ABSTRACT: Molecular tools for fluorescent imaging of specific compartments in cells are essential for understanding the function and activity of cells. Here, we report the synthesis of a series of pyridyl- and thienyl-substituted phospholes and the evaluation of these dyes for fluorescent imaging of cells. The thienyl-substituted phospholes proved to be successful for staining of cultured normal and malignant cells due to their fluorescent properties and low toxicity. Co-staining experiments demonstrated that these probes target lipid droplets, which are, lipid-storage organelles found in the cytosol of nearly all cell types. Our findings confirm that thienyl-substituted phospholes can be utilized as fluorescent tools for vital staining of cells, and we foresee that these fluorescent dyes might be u

SUBMITTER: Oberg E 

PROVIDER: S-EPMC5403830 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature