Loss of hepatic DEPTOR alters the metabolic transition to fasting.

ABSTRACT:

Objective

The mechanistic target of rapamycin (mTOR) is a serine/threonine kinase that functions into distinct protein complexes (mTORC1 and mTORC2) that regulates growth and metabolism. DEP-domain containing mTOR-interacting protein (DEPTOR) is part of these complexes and is known to reduce their activity. Whether DEPTOR loss affects metabolism and organismal growth in vivo has never been tested.

Methods

We have generated a conditional transgenic mouse allowing the tissue-specific deletion of DEPTOR. This model was crossed with CMV-cre mice or Albumin-cre mice to generate either whole-body or liver-specific DEPTOR knockout (KO) mice.

Results

Whole-body DEPTOR KO mice are viable, fertile, normal in size, and do not display any gross physical and metabolic abnor

SUBMITTER: Caron A 

PROVIDER: S-EPMC5404102 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature