DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli.

ABSTRACT:

Background

During infection of the urinary tract, uropathogenic Escherichia coli (UPEC) are exposed to different environments, such as human urine and the intracellular environments of bladder epithelial cells. Each environment elicits a distinct bacterial environment-specific transcriptional response. We combined differential fluorescence induction (DFI) with next-generation sequencing, collectively termed DFI-seq, to identify differentially expressed genes in UPEC strain UTI89 during growth in human urine and bladder cells.

Results

DFI-seq eliminates the need for iterative cell sorting of the bacterial library and yields a genome-wide view of gene expression. By analysing the gene expression of UPEC in human urine we found that genes involved in amino acid biosynthesis wer

SUBMITTER: Madelung M 

PROVIDER: S-EPMC5404293 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature