Aberrant DNA methylation and expression of SPDEF and FOXA2 in airway epithelium of patients with COPD.

ABSTRACT:

Background

Goblet cell metaplasia, a common feature of chronic obstructive pulmonary disease (COPD), is associated with mucus hypersecretion which contributes to the morbidity and mortality among patients. Transcription factors SAM-pointed domain-containing Ets-like factor (SPDEF) and forkhead box protein A2 (FOXA2) regulate goblet cell differentiation. This study aimed to (1) investigate DNA methylation and expression of SPDEF and FOXA2 during goblet cell differentiation and (2) compare this in airway epithelial cells from patients with COPD and controls during mucociliary differentiation.

Methods

To assess DNA methylation and expression of SPDEF and FOXA2 during goblet cell differentiation, primary airway epithelial cells, isolated from trachea

SUBMITTER: Song J 

PROVIDER: S-EPMC5404321 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature